Conformational studies of hemoglobins using intrinsic fluorescence measurements.

Single-molecule fluorescence studies of protein folding and conformational dynamics.

Studies on Invertebrate Hemoglobins (erythrocruorins)

1. Two high molecular invertebrate hemoglobins (the erythrocruorins of Lumbricus terrestris and of Nereis virens) as well as the low molecular erythrocruorin of Glycera dibranchiata Ehlers were studied. Their physical chemical properties were compared with those of vertebrate hemoglobin. 2. The hemin of the blood pigment of Glycera dibranchiata Ehlers was shown to be identical with that of vert...

comparative dna interaction studies of antiviral drug, zidovudine and its complex using different instrumental methods

هدف از این مطالعه بررسی امکان استفاده از داروهای شناخته شده در درمان سایر بیماریها به عنوان داروهای ضد سرطان است. همچنین با استفاده از این داروها در ساختمان کمپلکس فلز می توان شاخص های دارویی بدست آمده را بررسی نمود. داروی ضد ویروس ایدز(hiv)به نام زیدوودین(azt)انتخاب و.کمپلکس.محلول.در.آب[pt(azt)2]cl2سنتزو به روشهای مختلف فیزیکی و شیمیایی شناسایی گردید. بر هم کنش مقایسه ای این دارو و کمپلکس پلا...

Studies on ATP-diphosphohydrolase nucleotide-binding sites by intrinsic fluorescence.

Potato apyrase, a soluble ATP-diphosphohydrolase, was purified to homogeneity from several clonal varieties of Solanum tuberosum. Depending on the source of the enzyme, differences in kinetic and physicochemical properties have been described, which cannot be explained by the amino acid residues present in the active site. In order to understand the different kinetic behavior of the Pimpernel (...

Intrinsic fluorescence studies on saccharide binding to Artocarpus integrifolia lectin.

The combining region of Artocarpus integrifolia lectin has been studied by using the ligand-induced changes in the fluorescence of the lectin. The saccharide binding properties of the lectin show that C-1, C-2, C-4, and C-6 hydroxyl groups of D-galactose are important loci for sugar binding. The alpha-anomer of galactose binds more strongly than its beta-counterpart. Inversion in the configurat...